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KMID : 1001920040350040422
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Journal of Korean Neurosurgical Society
2004 Volume.35 No. 4 p.422 ~ p.426
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Development of Biodegradable Polymer Releasing Anticancer Drug for Intratumoral Chemotherapy of Brain Tumor
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Cho Yoon-Chul
Cho Han-Jin
An Jeong-Ho
Lee Won-Chung
Yoon Soo-Han
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Abstract
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Objective : The authors present two polymers as carriers of anti-neoplastic agents for intratumoral chemotherapy. We investigated in vitro tumor cytotoxicity againt C6 glioma cells with 20ul cis-platinum(CDDP)-polymer.
Methods : We firstly developed new two thermosensitive sol-gel reversible polymer(poly-2 and poly-6) by random copolyerization from ethylene glycol and caprolactone polymer that is sol state over 50C but changed gradually to gel less than 50C We evaluated the time-related release profiles of 10mg cisplatin from CDDP-Poly 2 and CDDPPoly 6 in 25ml of pH 7.4 phosphate buffer saline using UV spectrophotometer during 31 days. We cultured C6 glioma cell line in 10cm round plates for 2 days and added 20Q of CDDP-polymer containing 30uM of CDDP into the center of each plate. We counted C6 cell number every 12 hours for 72 hours at same 3 sites of each 6 well plates.
Results : UV spevtrophotometry showed total 12.6% of CDDP released from CDDP-poly2 and total 56.9% of CDDP from CDDP-poly6 on 31 days. Both carrier polymer showed total released amount of CDDP directly correlated with time without initial dumping effect. The CDDP released from 204 of CDDP-poly2 and CDDP-poly6 in 48 hours showed cytotoxicity to Co glioma cells more than 50% in vitro cell culture system.
Conclusion : This results suggest that less than 20Q CDDP-polymer could be applied in vivo brain tumor model to show significant cytotoxicity.
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KEYWORD
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Chemotherapy, Polymer, Drug carrier, Glioma Cisplatin
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